Coding

Part:BBa_K3452897:Design

Designed by: Haoran Hu   Group: iGEM20_CSU_CHINA   (2020-10-26)


light-oxygen-voltage (LOV) domain


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1053
    Illegal PstI site found at 1334
    Illegal PstI site found at 1495
    Illegal PstI site found at 1790
    Illegal PstI site found at 2395
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1334
    Illegal PstI site found at 1495
    Illegal PstI site found at 1790
    Illegal PstI site found at 2395
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1189
    Illegal BamHI site found at 1830
    Illegal XhoI site found at 1231
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1053
    Illegal PstI site found at 1334
    Illegal PstI site found at 1495
    Illegal PstI site found at 1790
    Illegal PstI site found at 2395
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1053
    Illegal PstI site found at 1334
    Illegal PstI site found at 1495
    Illegal PstI site found at 1790
    Illegal PstI site found at 2395
    Illegal NgoMIV site found at 2931
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2188
    Illegal SapI.rc site found at 1294
    Illegal SapI.rc site found at 2604


Design Notes

codon optimization was accomplished to fit to the Synechocystis


Source

Arabidopsis thaliana

References